Exonuclease III-Generated Series of Homologous Competitor DNA Fragments for Competitive PCR
نویسندگان
چکیده
منابع مشابه
Competitive RT-PCR: Preparation of Competitor RNA.
INTRODUCTION The first step in competitive RT-PCR is the synthesis and purification of the synthetic competitor. This is an RNA molecule designed to be reverse-transcribed and PCR-amplified with the same efficiency as the endogenous transcript of interest. Once the competitor molecule has been prepared, as described in this protocol, competitive PCR can be carried out, as described in Comptetit...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2000
ISSN: 0736-6205,1940-9818
DOI: 10.2144/00292bm05